Jackson二抗|515-035-003*

通過免疫親和層析從抗血清分離全I(xiàn)gG抗體作為完整分子。 它們具有Fc部分和兩個(gè)通過二硫鍵連接在一起的抗原結(jié)合Fab部分，因此它們是二價(jià)的。 據(jù)報(bào)道平均分子量約為160kDa。 整個(gè)IgG形式的抗體適用于大多數(shù)免疫檢測程序，并且是成本效益的。

基于免疫電泳和/或ELISA，抗體與全分子小鼠IgG反應(yīng)。 它還與其他小鼠免疫球蛋白的輕鏈反應(yīng)。 未檢測到針對非免疫球蛋白血清蛋白的抗體。 抗體可以與來自其他物種的免疫球蛋白交叉反應(yīng)。

Jackson二抗|515-035-003*

Physical State: Freeze-dried solid 
Storage:

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. 
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. 
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) 

Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry
1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000 - 1:200,000 for Western blotting with ECL substrates

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.