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Flow Tech公司MICROFIL® 造影劑現(xiàn)貨

  • 發(fā)布日期:2018-03-13      瀏覽次數(shù):2175
    • MICROFIL® Silicone Rubber Injection Compounds造影劑

      MV-112 MV-117 MV-120 MV-122 MV-130 MV-132 MV-Diluent

      http://www.flowtech-inc。。com

       

      About MICROFIL®

      MICROFIL® compounds will fill and opacify microvascular and other spaces of non-surviving animals and post-mortem tissue under physiological injection pressure. The continuous, closed vascular system lends itself to flow through injection or perfusion techniques. Following injection, MICROFIL compounds cure to form a three-dimensional cast of the vasculature.
      MICROFIL® MV-series compounds are available in five radiopaque colors, as well as clear. MV-series compounds require either an alcohol-methyl salicylate or glycerin clearing sequence, whereby the refractive index of the clearing solution is the same as the refractive index of the tissue. This allows for microscopic examination of a selected vascular bed.
      MICROFIL® CP-101 compound is intended for use in cast-corrosion techniques, and is designed for filling large blood vessels (greater than 100 microns). Although the CP-101 compound will fill capillaries, these vessels fragment when the supporting tissue is removed through exposure to a potassium hydroxide solution. When cured, MICROFIL® CP-101 is a milky white in color. Casts made using CP-101 will maintain their dimensional accuracy indefiniy.
      Advantages offered with MICROFIL® compounds over previously available rubber injection materials include:
      · Complete filling with minimal shrinkage, to enhance vessel continuity and to produce in cleared preparations a vivid, optically cleared specimen that allows a precise study of the microcirculation.
      · Color diversity, to provide delineation within the circulatory tree for microscopic examination and photographic illustration.

      Areas of Investigation

      In physiology, MICROFIL® visualization provides a means for establishing the precise vascular architecture of specific organs, allowing comparison between normal and abnormal structure.
      In surgery, visualization of the microcirculation and microanatomy is leading to improved surgical techniques in the repair of nerves, tendons, and blood vessels.
      In gastrointestinal research, MICROFIL® compounds characterize and describe changes in vascular patterns associated with several pathological conditions.
      Injected specimens, when preserved in methyl salicylate or glycerin, also serve as a definitive teaching adjunct.

      MV-Series Mixing Procedure

      To achieve a viscosity level suitable for injection of the microcirculation, it is necessary to blend the MV compound with an equal quantity (by weight) of MV-Diluent. Volume mixing requires 5ml of diluent for every 4ml of compound. The mixture of compound and diluent is catalyzed with 5% (by weight or volume) of MV Curing Agent. Viscosity ranges from 20 to 30 centipoise. Working time is 20 minutes and begins with the addition of curing agent.



       

      Notes

      1. Viscosity measured with a Brookfield Model LVF Viscometer, with a No. 2 spindle at 30RPM.
      2. Gel time measured on a blend (by weight) of one part MV compound and one part MV-Diluent followed by addition of 5% MV Curing Agent. Gel time is time required for mixture to cease flowing.
      3. Shelf life is in excess of four months. For materials held beyond four months, it is possible to run the following static test to determine acceptability:
      a. Mix 5 grams of MV compound with 5 grams of MV-Diluent in a vial.
      b. Add 10 drops (medicine dropper) of MV Curing Agent.
      c. Cap, shake, and refrigerate overnight.
      d. The mixture should gel in the vial after this procedure to assure a cure in subsequent animal injections.
      Catalyzed mixtures will form an elastomeric gel after 90 minutes at room temperature. Curing takes place with non-exothermic cross-linking and minimal volume change.
      It has been possible to refrigerate specimens immediay after injection and still obtain complete cure after overnight aging. The procedure decreases odor level for subsequent sectioning.

      Perfusion Techniques

      Two techniques of tissue clearing are described below. Alcohol-methyl salicylate clearing produces a stiffer tissue, which, from an aesthetic point, provides a pleasing view for gross observation. Glycerin clearing produces a more flexible tissue, allowing easier manipulation for a given vessel.

      Alcohol-methyl salicylate clearing

      Non-wetting features of MICROFIL® compounds prevent any interaction with blood. Therefore, in the non-surviving animal, a selected vascular bed can be readily perfused without prior washout of blood. Heparinization to maintain blood fluidity, however, has been used to realize improved injection preparations.
      For the injection of blood vessels from vascular beds removed post-mortem, washout of clotted blood with saline is advisable.
      Selected vascular beds are perfused through their accessible artery and drained through a similar vein. Infusion pressures will vary with the animal’s mean systemic pressure. For organs from the dog, cat, rat, and man, a pressure of 150mm.Hg for arterial filling has been used, and 25 to 50mm.Hg for venous fillings.

      Flow Tech公司MICROFIL® 造影劑現(xiàn)貨

      Flow Tech,Inc.是一家馬薩諸塞州公司,于1996年從Boulder公司搬遷,我們能為您提供便利和專業(yè)的服務(wù),在大多數(shù)情況下,您可以在收到后的一個工作日內(nèi)發(fā)貨。

      MICROFIL®化合物設(shè)計(jì)用于在生理壓力下填充和遮蓋非生存動物和尸體后的微血管和其他空間。這一創(chuàng)新產(chǎn)品已經(jīng)在美國和的高校,醫(yī)院和其他研究機(jī)構(gòu)使用了三十多年。

      關(guān)于MICROFIL®

      MICROFIL®化合物將在生理注射壓力下填充和不透明非生存動物和死后組織的微血管和其他空間。連續(xù)閉合的血管系統(tǒng)本身可以通過注射或灌注技術(shù)流動。注射后,MICROFIL化合物固化形成脈管系統(tǒng)的三維鑄型。

      MICROFIL®MV系列化合物有五種不透射線的顏色以及透明度。MV系列化合物需要水楊酸甲酯或甘油清除順序,由此清除溶液的折射率與組織的折射率相同。這允許對所選擇的血管床進(jìn)行顯微鏡檢查。

      MICROFIL®CP-101化合物適用于鑄造腐蝕技術(shù),用于填充大血管(大于100微米)。雖然CP-101化合物將填充毛細(xì)血管,但是當(dāng)通過暴露于氫氧化鉀溶液除去支持組織時,這些血管碎片。固化時,MICROFIL®CP-101是乳白色的白色。使用CP-101制作的鑄件將無限期地保持其尺寸精度。

      MICROFIL®化合物優(yōu)于以前提供的橡膠注射材料的優(yōu)勢包括:

      • *填充zui小的收縮,增強(qiáng)血管連續(xù)性,并在清除的制劑中產(chǎn)生一個生動的,光學(xué)清除的樣品,允許研究微循環(huán)。
      • 色彩多樣性,在循環(huán)樹中提供用于顯微鏡檢查和照相插圖的描繪。

      Flow Tech公司MICROFIL® 造影劑現(xiàn)貨

      Flow Tech公司MICROFIL® 造影劑現(xiàn)貨

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